CcpA forms complexes with CodY and RpoA in Bacillus subtilis
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چکیده
منابع مشابه
Interaction of Bacillus subtilis CodY with GTP.
Many of the adaptive mechanisms that allow Bacillus subtilis to adjust to changes in nutrient availability are controlled by CodY. Binding of CodY to its target genes is stimulated by interaction with its effectors, GTP and the branched-chain amino acids (BCAAs). Upon nutrient limitation, intracellular pools of these effectors are depleted and CodY can no longer repress genes required for adapt...
متن کاملBacillus subtilis CodY operators contain overlapping CodY binding sites.
CodY is a global transcriptional regulator that is activated by branched-chain amino acids. A palindromic 15-bp sequence motif, AATTTTCNGAAAATT, is associated with CodY DNA binding. A gel mobility shift assay was used to examine the effect of pH on the binding of Bacillus subtilis CodY to the hutPp and ureAp(3) promoters. CodY at pH 6.0 has higher affinity for DNA, more enhanced activation by i...
متن کاملCcpA-independent carbon catabolite repression in Bacillus subtilis.
The past decade has witnessed an exiting unveiling of numerous molecular mechanisms that characterize signal transduction by protein-protein interaction. The recent findings encouraged an increasing effort to understand the sequential metabolism of different sugars available as energy sources at the same time. It seems probable that at least three principle mechanisms which act together or sepa...
متن کاملCcpA-dependent regulation of Bacillus subtilis glutamate dehydrogenase gene expression.
The Bacillus subtilis rocG gene, encoding catabolic glutamate dehydrogenase, was found to be subject to direct CcpA-dependent glucose repression. The effect of CcpA required the presence of both the HPr and Crh proteins. The primary CcpA binding site was identified by mutational analysis and DNase I footprinting. In the absence of inducers of the Roc pathway, rocG was still expressed at a low l...
متن کاملCcpA - Mediated Catabolite Activation of the Bacillus subtilis ilv - leu Operon 1 and Its Negation by Either CodY - or TnrA - Mediated Negative Regulation
CcpA-Mediated Catabolite Activation of the Bacillus subtilis ilv-leu Operon 1 and Its Negation by Either CodYor TnrA-Mediated Negative Regulation 2 3 4 Yasutaro Fujita*, Takenori Satomura, Shigeo Tojo, and Kazutake Hirooka 5 6 7 Department of Biotechnology, Faculty of Life Science and Biotechnology, Fukuyama 8 University, 985 Sanzo, Higashimura-cho, Fukuyama-shi, Hiroshima 729-0292, Japan 9 10 ...
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ژورنال
عنوان ژورنال: FEBS Journal
سال: 2012
ISSN: 1742-464X
DOI: 10.1111/j.1742-4658.2012.08604.x